[…] “VC seems to influence the structure and function of epidermis, especially by controlling the growth of epidermal cells. In this study, we investigated whether it promotes cell proliferation and differentiation via epigenetic changes,” explains Dr. Ishigami, while talking about this study.

To investigate how VC affects skin regeneration, the team used human epidermal equivalents, which are laboratory-grown models that closely mimic real human skin. In this model, skin cells are exposed to air on the surface while being nourished from underneath by a liquid nutrient medium, replicating the way human skin receives nutrients from underlying blood vessels while remaining exposed to the external environment.

The researchers used this model and applied VC at 1.0 and 0.1 mM — concentrations comparable to those typically transported from the bloodstream into the epidermis. On assessing its effect, they found that VC-treated skin showed a thicker epidermal cell layer without significantly affecting the stratum corneum (the outer layer composed of dead cells) on day seven. By day 14, the inner layer was even thicker, and the outer layer was found to be thinner, suggesting that VC promotes the formation and division of keratinocytes. Samples treated with VC showed increased cell proliferation, demonstrated by a higher number of Ki-67-positive cells — a protein marker present in the nucleus of actively dividing cells.

Importantly, the study revealed that VC helps skin cells grow by reactivating genes associated with cell proliferation. It does so by promoting the removal of methyl groups from DNA, in a process known as DNA demethylation. When DNA is methylated, methyl groups attach to cytosine bases, which can prevent the DNA from being transcribed or read, thereby suppressing gene activity. Conversely, by promoting DNA demethylation, VC promotes gene expression and helps cells to grow, multiply, and differentiate.

The study suggests that VC supports active DNA demethylation by sustaining the function of TET enzymes (ten-eleven translocation enzymes), which regulate gene activity. These enzymes convert 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine (5-hmC), a process in which Fe²⁺ is oxidized to Fe³⁺. VC helps maintain TET enzyme activity by donating electrons to regenerate Fe²⁺ from Fe³⁺, enabling continued DNA demethylation.

The researchers further identified over 10,138 hypomethylated differentially methylated regions in VC-treated skin and observed a 1.6- to 75.2-fold increase in the expression of 12 key proliferation-related genes. When a TET enzyme inhibitor was applied, these effects were reversed, confirming that VC functions through TET-mediated DNA demethylation.

These findings reveal how VC promotes skin renewal by triggering genetic pathways involved in growth and repair. This suggests that VC may be particularly helpful for older adults or those with damaged or thinning skin, boosting the skin’s natural capacity to regenerate and strengthen itself.

“We found that VC helps thicken the skin by encouraging keratinocyte proliferation through DNA demethylation, making it a promising treatment for thinning skin, especially in older adults,” concludes Dr. Ishigami.

This study was supported by grants from the Japan Society for the Promotion of Science (JSPS) KAKENHI: grant number 19K05902.

Source: Vitamin C flips your skin’s “youth genes,” reversing age-related thinning | ScienceDaily

How much you need to take to achieve this effect is however a mystery.

Researchers at University of California San Diego School of Medicine have developed a gene therapy for Alzheimer’s disease that could help protect the brain from damage and preserve cognitive function. Unlike existing treatments for Alzheimer’s that target unhealthy protein deposits in the brain, the new approach could help address the root cause of Alzheimer’s disease by influencing the behavior of brain cells themselves.

Alzheimer’s disease affects millions of people around the world and occurs when abnormal proteins build up in the brain, leading to the death of brain cells and declines in cognitive function and memory. While current treatments can manage symptoms of Alzheimer’s, the new gene therapy aims to halt or even reverse disease progression.

Studying mice, the researchers found that delivering the treatment at the symptomatic stage of the disease preserved hippocampal-dependent memory, a critical aspect of cognitive function that is often impaired in Alzheimer’s patients. Compared to healthy mice of the same age, the treated mice also had a similar pattern of gene expression, suggesting that the treatment has the potential to alter the behavior of diseased cells to restore them to a healthier state.

While further studies will be required to translate these findings into human clinical trials, the gene therapy offers a unique and promising approach to mitigating cognitive decline and promoting brain health.

The study, published in Signal Transduction and Targeted Therapy, was led by senior author Brian Head, Ph.D., professor of anesthesiology at UC San Diego School of Medicine and Veterans Affairs research career scientist, and co-senior author Shanshan Wang, M.D. Ph.D., an assistant professor of anesthesiology at UC San Diego School of Medicine. The gene therapy technology was licensed by UC San Diego to Eikonoklastes Therapeutics in 2021. Eikonoklastes was granted Orphan Drug Designation (ODD) by the FDA for the use of the patented gene therapy in amyotrophic lateral sclerosis (ALS) also known as Lou Gehrig’s disease.

Source: Brain reboot: Gene therapy reverses Alzheimer’s memory loss in mice | ScienceDaily

Swarms of tiny robots, each no larger than a speck of dust, could be deployed to cure stubborn infected sinuses before being blown out through the nose into a tissue, researchers have claimed.

The micro-robots are a fraction of the width of a human hair and have been inserted successfully into animal sinuses in pre-clinical trials by researchers at universities in China and Hong Kong.

Swarms are injected into the sinus cavity via a duct threaded through the nostril and guided to their target by electromagnetism, where they can be made to heat up and catalyse chemical reactions to wipe out bacterial infections. There are hopes the precisely targeted technology could eventually reduce reliance on antibiotics and other generalised medicines.

The tiny devices are part of the expanding field of micro- and nano-robots for use in medicine. They have also been developed to deliver drugs and to remove bacteria from medical implants such as stents and hernia meshes.

Experts believe they could be in clinical use for treating infections in bladders, intestines and sinuses in five to 10 years. Scientists in China, Switzerland, the US and the UK are developing more sophisticated versions capable of moving through the bloodstream.

The latest development came from a collaboration of academics at the Chinese University in Hong Kong, and universities in Guangxi, Shenzhen, Jiangsu, Yangzhou and Macau.

Researchers in the emerging field acknowledge risks include some of the tiny micro-robots being left behind after treatment which could cause longer-term side effects.

[…]

The study, published in Science Robotics, showed the robots were capable of eradicating bacteria from pig sinuses and could clear infections in live rabbits with “no obvious tissue damage”.

The researchers have produced a model of how the technology could work on a human being, with the robot swarms being deployed in operating theatre conditions, allowing doctors to see their progress by using X-rays. Future applications could include tackling bacterial infections of the respiratory tract, stomach, intestine, bladder and urethra, they suggested.

“Our proposed micro-robotic therapeutic platform offers the advantages of non-invasiveness, minimal resistance, and drug-free intervention,” they said.

[…]

Source: Swarms of tiny nose robots could clear infected sinuses, researchers say | Medical research | The Guardian

Bacteria can be used to turn plastic waste into painkillers, researchers have found, opening up the possibility of a more sustainable process for producing the drugs.

Chemists have discovered E coli can be used to create paracetamol, also known as acetaminophen, from a material produced in the laboratory from plastic bottles.

“People don’t realise that paracetamol comes from oil currently,” said Prof Stephen Wallace, the lead author of the research from the University of Edinburgh. “What this technology shows is that by merging chemistry and biology in this way for the first time, we can make paracetamol more sustainably and clean up plastic waste from the environment at the same time.”

Writing in the journal Nature Chemistry, Wallace and colleagues report how they discovered that a type of chemical reaction called a Lossen rearrangement, a process that has never been seen in nature, was biocompatible. In other words, it could be carried out in the presence of living cells without harming them.

The team made their discovery when they took polyethylene terephthalate (PET) – a type of plastic often found in food packaging and bottles – and, using sustainable chemical methods, converted it into a new material.

When the researchers incubated this material with a harmless strain of E coli they found it was converted into another substance known as Paba in a process that must have involved a Lossen rearrangement.

Crucially, while the Lossen rearrangement typically involves harsh laboratory conditions, it occurred spontaneously in the presence of the E coli, with the researchers discovering it was catalysed by phosphate within the cells themselves.

The team add that Paba is an essential substance that bacteria need for growth, in particular the synthesis of DNA, and is usually made within the cell from other substances. However, the E coli used in the experiments was genetically modified to block these pathways, meaning the bacteria had to use the PET-based material.

The researchers say the results are exciting as they suggest plastic waste can be converted into biological material.

“It is a way to just completely hoover up plastic waste,” said Wallace.

The researchers then genetically modified the E coli further, inserting two genes – one from mushrooms and one from soil bacteria – that enabled the bacteria to convert PABA into paracetamol.

The team say that by using this form of E coli they were able to turn the PET-based starting material into paracetamol in under 24 hours, with low emissions and a yield of up to 92%.

While further work would be needed to produce paracetamol in this way at commercial levels, the results could have a practical application.

“It enables, for the first time, a pathway from plastic waste to paracetamol, which is not possible using biology alone, and it’s not possible using chemistry alone,” Wallace said.

Source: Scientists use bacteria to turn plastic waste into paracetamol | Drugs | The Guardian

  Weather-related stressors on healthy brain development has become an important topic in recent years. Notably, prenatal stress exposure to natural disasters may disrupt child neurodevelopment, with recent research exploring its impact on child brain morphology. Prenatal exposure to extreme weather events, such as ambient heat, may also affect child brain morphology. The basal ganglia, while historically related to motor ability, has gained increasing attention for its role in various non-motor functions, such as emotion regulation. Leveraging an existing cohort with and without prenatal exposure to Superstorm Sandy (SS), a category 3 hurricane at its peak, this study aims to investigate how prenatal exposure to both a natural disaster and extreme ambient heat impacts this important subcortical region.

[…]

Conclusions: Prenatal exposure to SS impacted child brain development. Extreme heat amplified this risk via increased and reduced brain volume from different basal ganglia subregions. Alongside promoting initiatives to combat climate change, increasing awareness of the potential dangers of exposure to extreme climate events for pregnant individuals is vital for protecting long-term child brain development.

Source: Prenatal exposure to extreme ambient heat may amplify the adverse impact of Superstorm Sandy on basal ganglia volume among school-aged children – PubMed